Glucuronyl C5-epimerase, DNA encoding the same and uses thereof
AssigneesBiotie Therapies, Inc., Inalco S.p.A.
InventorsMarkku Jalkanen, Kamel El Darwish, Ulf Lindahl, Jin-ping Li
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Abstract
The invention is directed to a novel purified mouse C5-epimerase, fragments thereof, nucleic acids encoding the same and the recombinant production thereof. The invention is also directed to fragments of such epimerase, especially N-terminal fragments that are useful in fusion protein constructs to enhance the activity of recombinantly-produced heterologous epimerase enzymes.
Claims15
An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide, the amino acid sequence of which is at least 95% identical to
- amino acids 1-618 of FIG. 3. (SEQ ID No. 2),
- wherein said amino acid sequence encodes a polypeptide that has C5-epimerase enzymatic activity.
A method of increasing the activity of a C5-epimerase, said method comprising:
- (a) providing a first polynucleotide comprising a nucleotide sequence encoding a polypeptide, the amino acid sequence of which is at least 80% identical to a reference amino acid sequence selected from the group consisting of amino acids 35 to 154 of FIG. 3 and amino acids 34 to 154 of FIG. 3;
- (b) attaching said first polynucleotide of (a) in frame to the N-terminus of a second polynucleotide encoding a C5-epimerase;
- and
- (c) expressing the fusion polynucleotide,
- wherein the C5-epimerase that is encoded by said fusion polynucleotide has an increased C5-epimerase activity when compared to the C5-epimerase activity of said polypeptide that is encoded by said second polynucleotide.
Dependencies are inferred from the claim text.