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US 6,887,691 B2Granted
DNA coding for protein which confers on bacterium Escherichia coli resistance to L-homoserine, and method for producing L-amino acids
AssigneeAjinomoto Co., Inc.
InventorsVitaly Arkadievich Livshits, Natalya Pavlovna Zakataeva, Vladimir Venyamiovich Aleoshin, Alla Valentinovna Balareova, Irina Lvovna Tokhmakova
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Abstract
A bacterium which has an ability to produce an amino acid and in which a novel gene (rhtB) coding for a protein having an activity of making a bacterium having the protein L-homoserine-resistant is enhanced, is cultivated in a culture medium to produce and accumulate the amino acid in the medium, and the amino acid is recovered from the medium.
Claims5
A method for producing an amino acid selected from the group consisting of L-threonine, L-homoserine, L-alanine, L-isoleucine and L-valine, comprising:
- cultivating a bacterium which has an ability to produce and cause accumulation of the amino acid in a culture medium, and
- recovering the amino acid from the medium,
- wherein said bacterium is a bacterium belonging to the genus Escherichia, wherein L-homoserine resistance of said bacterium is enhanced by amplifying a copy number of a DNA in said bacterium, wherein said DNA codes for a protein comprising the amino acid sequence shown in SEQ ID NO:2.
Dependencies are inferred from the claim text.