Immobilized carbohydrate biosensor
OwnerKurt Nilsson, Carl-Fredrik Mandeniusinventors
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Abstract
The present invention refers to a biosensor in which an immobolized carbohydrate or a derivative thereof is used to generate a detectable signal when a protein, a virus or a cell is bound to the carbohydrate surface. The sensor is an optical sensor, a piezoelectric sensor, an electrochemical electrode or a thermistor. A method of binding carbohydrates to a gold surface is also described.
Claims9
An immobilized carbohydrate derivative biosensor, comprising:
- a surface;
- and
- a carbohydrate derivative, bound to the surface, which specifically binds to at least one biomolecule in a sample wherein the carbohydrate derivative comprises a fragment of a carbohydrate sequence found in a glycoprotein or a glyco lipid which is an oligosaccharide modified in the reducing end with an aglycon selected from the group consisting of —OEtSEtCONHNH2 and OEtSPhNH2.
An immobilized carbohydrate derivative biosensor, comprising:
- a surface;
- and
- a carbohydrate derivative, bound to the surface, which specifically binds to at least one biomolecule in a sample wherein the carbohydrate derivative is a derivative in which the carbohydrate is modified in the reducing end with an O-, N-, C- or S-glycosidically bound aglycon, and said
- glycosidically bound aglycon comprises a structure corresponding to a formula —R—X, wherein the biosensor corresponds to the formula:
- carbohydrate-R—NH—CO—CH2CH2—S-biosensor surface.
An immobilized carbohydrate derivative biosensor, comprising:
- a surface;
- and
- a carbohydrate derivative, bound to the surface, which specifically binds to at least one biomolecule in a sample wherein the carbohydrate derivative is a derivative in which the carbohydrate is modified in the reducing end with an O—, N—, C- or S-glycosidically bound aglycon, comprising a structure corresponding to a formula —R—X, and wherein the biosensor corresponds to the formula carbohydrate-R—X-protein-NH—CO—CH2—CH2—S-biosensor surface wherein X is a member selected from the group consisting of —S—, —NH—CO—, CO—NH—, —NH—, and —N=N—.
An immobilized carbohydrate derivative biosensor, comprising:
- a surface;
- and
- a carbohydrate derivative, bound to the surface, which specifically binds to at least one biomolecule in a sample,
- wherein the surface comprises silica coated with a gold layer modified with mercaptopropionic acid by dipping the surface in a 5 mM solution of the acid.
Dependencies are inferred from the claim text.