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US 6,887,498 B2Granted
Method of making Bowman-Birk inhibitor product
AssigneeSolae, LLC
InventorsArthur H. Konwinski, Bernard F. Szuhaj
Life of this patent
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Abstract
A method for preparing a Bowman-Birk inhibitor (BBI) product from soybeans is provided. The product produced in accordance with the method of the invention and compositions including the product are also provided. BBI product produced in accordance with the invention is a significantly improved inhibiter of malignant cell transformation and methods for its administration to prevent or inhibit progression of cancer are provided.
Claims15
A method for making a Bowman-Birk product comprising:
- introducing and mixing acetone with acid extracted solubles from defatted soybeans wherein the amount of acetone is about two times (by weight) the amount of acid extracted solubles;
- allowing the acetone and acid extracted solubles mixture to settle for at least 1 hour to form a precipitate;
- separating the precipitate from the liquid by decantation;
- introducing and mixing acetone with the separated precipitate wherein the amount of acetone is about one half of the amount by weight utilized in the first mixing step;
- allowing the acetone and separated precipitate mixture to settle;
- separating the precipitate from the liquid by decantation;
- vacuum filtering the separated precipitate and drying the precipitate by air drying;
- dispersing the air dried precipitate in water;
- filtering the dispersion to remove insoluble materials;
- and spray drying the dispersion.
7 more elements
A method for making a Bowman-Birk inhibitor concentrate without alcohol extraction comprising:
- slurrying defatted soybean flakes with water;
- adjusting the pH of said slurry to about 4-6;
- mixing said pH adjusted slurry;
- separating soluble material from said pH adjusted slurry;
- mixing acetone with the soluble material and allowing the mixture to settle for at least 1 hour to form a precipitate, wherein the amount of acetone is about two times (by weight) the amount of the soluble material;
- separating the precipitate from the liquid by decantation;
- mixing acetone with said precipitate, wherein the amount of acetone is about one half the amount by weight utilized in the first mixing step
- separating the precipitate from the liquid by decantation;
- vacuum filtering the separated precipitate and drying the precipitate by air drying;
- dispersing the air dried precipitate in water;
- filtering the dispersion to remove insoluble materials;
- and spray drying the dispersion.
9 more elements
Dependencies are inferred from the claim text.