US 6,887,430 B1 · Apparatus for immune analysis

Abstract

A total reflection cell has a total reflection prism on at least one surface thereof. A mixed solution containing a gold colloid labelled antibody which is adsorbed on a gold colloid is stored in the total reflection cell, and a sample solution containing an antigen causing antigen-antibody reaction with the antibody is added thereto for forming a gold colloid labelled immune complex. A measuring beam is introduced into the total reflection prism from an incident optical system at an angle θ of incidence causing total reflection and an outgoing beam from the total reflection prism is received by a measuring optical system, thereby measuring absorption by the gold colloid labelled immune complex and carrying out qualification and determination of the antigen.

Claims11

Dependent claims

An immunoassay apparatus comprising:

a total reflection cell having at least one total reflection prism consisting of a material having a large refractive index than a sample mixed solution containing an immune complex being labelled with a noble metal colloid, wherein said at least one total reflection prism is on at least one wall surface which defines a space in which said sample mixed solution is present;
an incident optical system configured to introduce a measuring beam of a wavelength in a range from visible to infrared regions into said at least one total reflection prism at an angle of incidence causing total reflection;
and
a measuring optical system configured to receive an outgoing beam from said at least one total reflection prism for measuring absorbance by said sample mixed solution.

1 more element

Dependencies are inferred from the claim text.

Apparatus for immune analysis

Life of this patent

Apparatus for immune analysis

Life of this patent

Abstract

Claims11