Method of identifying properties of substance with respect to human prostaglandin D2 receptors
AssigneeBML, Inc.
InventorsHiroyuki Hirai, Kazuyuki Ogawa, Kinya Nagata, Syoichi Takano
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Abstract
An object of the present invention is to provide a method of identifying a substance which acts on a newly found second human prostaglandin D2 receptor subtype that differs from the DP receptor, and is useful for treating or preventing various diseases. In order to attain the object, the present invention provides a method of identifying properties of a test substance with respect to a human prostaglandin D receptor, by establishing correlation between the effect of a test substance on human CRTH2 with the effect of the test substance on the human prostaglandin D receptor.
Claims11
A method for identifying a test substance which modulates human G-protein coupling receptor-like protein (CRTH2) activity, said method comprising:
- (a) contacting human CRTH2 with prostaglandin D2 (PGD2) in the presence of a test substance, and
- (b) comparing activity of the human CRTH2 of (a) with activity of a human CRTH2 contacted with PGD2 in the absence of the test substance, wherein when a change in activity is detected between the human CRTH2 contacted with PGD2 in the presence of the test substance and the human CRTH2 receptor contacted with PGD2 in the absence of the test substance, said test substance is determined to be a modulator of CRTH2 activity.
The method according to claim 1, further comprising assaying activity modulating effects of said test substance on DP receptor activity, wherein said assaying is performed by:
- (a) contacting DP receptor with prostaglandin D2 (PGD2) in the presence of the test substance identified in claim 5, and
- (b) comparing activity of the DP receptor of (a) with activity of a DP receptor contacted with PGD2 in the absence of the test substance, wherein when a change in activity is detected between the DP receptor contacted with PGD2 in the presence of the test substance and the DP receptor contacted with PGD2 in the absence of the test substance, said test substance is determined to possess activity modulating effects on DP receptor.
Dependencies are inferred from the claim text.