Formulation of stabilized cationic transfection agent(s) /nucleic acid particles
AssigneeAventis Pharma S.A.
InventorsJoël Crouzet, Bruno Pitard
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Abstract
The invention concerns a composition containing stabilized particles of cationic transfection agents/nucleic acid complexes characterized in that it includes besides said transfection agent and nucleic acid at least a non-ionic surfactant in sufficient amount for preventing the aggregation of the particles in course of time. In a preferred embodiment, the surfactant is a polyoxyalkylene or a derivative thereof.
Claims35
A composition comprising stabilized particles comprising a complex of at least one cationic transfection agent and a nucleic acid, and at least one nonionic surface-active agent, wherein said agent stabilizes the size of said particles to less than or equal to 160 nanometers;
- and wherein the at least one nonionic surface-active agent is chosen from (a) a polyoxyalkylene of the formula:
- HO(CH2CH2O)a(CH(CH3)CH2O)b(CH2CH2O)cH
- wherein a, b, and c are, independently, a number from 20 to 100, and
- (b) a polyethylene glycol comprising a dendritic benzyl polyether head.
Dependencies are inferred from the claim text.