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AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsHaruhiko Yokoi, Yukimasa Shiotsu, Noboru Konishi, Hideharu Anazawa, Tatsuya Tamaoki, Motoo Yamasaki, Yoko Kato, Kazuhisa Uchida, Kinya Yamashita
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The present invention relates to a fusion polypeptide which comprises a polypeptide having G-CSF activity and a polypeptide having TPO activity and DNA which codes for the fusion polypeptide, to a fusion polypeptide in which a polypeptide having G-CSF activity and a polypeptide having TPO activity are fused via a spacer peptide and DNA which codes for the fusion polypeptide and to a polypeptide in which the fusion polypeptide comprising a polypeptide having G-CSF activity and a polypeptide having TPO activity is chemically modified with a polyalkylene glycol derivative. It also relates to an anemia-treating composition containing the fusion polypeptide as an active ingredient.
A fusion polypeptide which comprises a human granulocyte colony stimulating factor polypeptide and c-mpl ligand polypeptide and has no mouse IL-3 activity as measured by inability to stimulate growth of Ba/F3 cells, wherein the polypeptide comprises the amino acid sequence shown in SEQ ID NO:1 or an amino acid sequence in which the amino acid nos. 155 to 328 of the amino acid sequence shown in SEQ ID NO:1 Is replaced by an amino acid sequence of SEQ ID NO:39 or by an amino acid sequence in which the amino acid nos. 155 to 328 of the amino acid sequence shown in SEQ ID NO:1 is replaced by an amino acid sequence of SEQ ID NO:46 having a set of substitutions selected from the group consisting of those of Table 2 a) to j) and l).
A fusion polypeptide which comprises a human granulocyte colony stimulating factor polypeptide and c-mpl ligand polypeptide and has no mouse IL-3 activity as measured by inability to stimulate growth of Ba/F3 cells, wherein the polypeptide comprises the amino acid sequence shown In SEQ ID NO:1 or an amino acid sequence in which the amino acid nos. 155 to 328 of the amino acid sequence shown in SEQ ID NO:1 is replaced by an amino acid sequence of SEQ ID NO:39 or by an amino acid sequence in which the amino acid nos. 155 to 328 of the amino acid sequence shown in SEQ ID NO:1 is replaced by an amino acid sequence of SEQ ID NO:46 having a set of substitutions selected from the group consisting of those of j) and l), and the human granulocyte colony stimulating factor polypeptide is fused via a spacer peptide to the c-mpl ligand polypeptide.
Dependencies are inferred from the claim text.