Integrated microfluidic disc
AssigneeGyros AB
InventorsNigel Eric Tooke, Per Andersson
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Abstract
Disclosed is a method for performing the steps of nucleic acid template purification, a thermocycling reaction and purification of the products of the thermocycling reaction characterised in that the steps take place sequentially in a microfluidic disc. Also disclosed is a microstructure for fluids comprising at least one inlet opening connected to a first chamber incorporating a means for purifying template nucleic acid which, in turn, is connected to a second chamber incorporating a means for a thermocycling reaction which, in turn, is connected to a third chamber incorporating a means for purifying products of the thermocycling reaction, and a microfluidic disc comprising a plurality of such microstructures.
Claims9
A method of processing a nucleic acid template on a microfluidic disc comprising the steps of:
- a) purifying the nucleic acid template;
- b) performing a thermocycling reaction on the purified nucleic acid template;
- and
- c) purifying a thermocycling reaction product from the thermocycling reaction,
- wherein step a-step c are performed sequentially on the microfluidic disc.
Dependencies are inferred from the claim text.