Chromatography method and a column material useful in said method
OwnerKnut Irgum, Camilla Viklundinventors
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Abstract
A novel sorbent suitable for use as a stationary phase in a chromatography column, the core of which consists of an organic polymer of synthetic or natural origin. Further, the carrier exhibits a plurality of covalently bonded non-aromatic zwitterionic groups on its surface. Additionally, the invention also relates to a method for purifying a particular biological macromolecule, such as a protein or a nucleic acid, by zwitterionic ion exchange chromatography as well as an ion exchange column suitable for use in the zwitterionic ion exchange chromatography.
Claims20
A sorbent suitable for use as a stationary phase in elution chromatography, the core of said sorbent consisting of an organic resin selected from the group consisting of polymers made from mono- or oligo-vinyl monomers and carbohydrates other than cellulose and wherein a plurality of non-aromatic zwitterionic groups are covalently bonded on the surface of said sorbent.
A sorbent, comprising:
- a core consisting of an organic resin selected from the group consisting of polymers made from mono- or oligo-vinyl monomers and carbohydrates other than cellulose;
- a sorbent surface;
- and
- a plurality of non-aromatic zwitterionic groups covalently bonded to said surface;
- and wherein said sorbent has selective sorption properties so that said sorbent can be used as a stationary phase in chromatographic separations.
A sorbent suitable for use as a stationary phase in elution chromatography, comprising:
- a core consisting of an organic resin selected from the group consisting of polymers made from mono- or oligo-vinyl monomers and carbohydrates other than cellulose;
- a sorbent surface;
- and
- a plurality of non-aromatic zwitterionic groups covalently bonded to the surface.
Dependencies are inferred from the claim text.