Gene encoding dihydrodipicolinate synthase from Bacillus methanolicus and methods of making lysine wing said gene
AssigneeAjinomoto Co., Inc.
InventorsNobuharu Tsujimoto, Hisashi Yasueda, Yoshio Kawahara, Shinichi Sugimoto
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Abstract
An Escherichia coil mutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed by using a chromosome gene library of Bacillus methanolicus, a transformant strain which can grow on a minimal medium is selected, and recombinant DNA containing DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase is obtained from the transformant.
Claims9
An isolated DNA which hybridizes under stringent conditions to nucleotides 1 to 924 of SEQ ID NO:
- 1, wherein said stringent conditions comprise washing at 60° C., 0.1×SSC and 0.1% SDS, and wherein said DNA codes for a protein having dihydrodipicolinate synthase activity.
An isolated DNA which codes for a protein having the amino acid sequence of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.