Tiling process for constructing a chemical array
AssigneeAgilent Technologies, Inc.
InventorCarol T. Schembri
Life of this patent
Estimating the term…
Abstract
A process of constructing an array of chemical moieties having the following steps: forming multiple discrete physical entities (tiles) from a substantially planar material having one or more species of chemical moiety attached thereto; and picking and placing the entities (tiles) stably on a support at spatially distinct ascertainable locations to form an array of chemical moieties. The formed array includes at least two species of chemical moiety and preferably from about 50 to about 1000 species. The claimed invention includes an array formed by this process.
Claims31
A process for constructing an chemical array comprising a plurality of species of bioorganic molecules in a predetermined arrangement, said process comprising the steps of:
- 1) for each species of said plurality of bioorganic molecules, constructing a batch of separate tiles by:
- (a) providing a unit of a substantially planar solid material having an attachment surface;
- (b) attaching said species of bioorganic molecule onto said attachment surface;
- and
- (c) subdividing said unit of substantially planar material to form a plurality of separate tiles, a surface of each of said separate tiles comprising a portion of said attachment surface;
- and
- 2) affixing separate tiles from the said batches of tiles in predetermined spatial positions on a support.
A process for constructing a chemical array comprising a plurality of species of nucleic acids in a predetermined arrangement, said process comprising the steps of:
- 1) for each species of said plurality of nucleic acids, constructing a batch of separate tiles by:
- (a) providing a unit of a substantially planar solid material having an attachment surface;
- (b) providing said species of nucleic acid onto said attachment surface so that they are attached thereto;
- and
- (c) subdividing said unit of substantially planar material to form a plurality of separate tiles, a surface of each of said separate tiles comprising a portion of said attachment surface;
- and
- 2) affixing separate tiles from the said batches of tiles in predetermined spatial positions on a support.
A process for constructing a chemical array comprising a plurality of species of proteins in a predetermined arrangement, said process comprising the steps of:
- 1) for each species of said plurality of proteins, constructing a batch of separate tiles by:
- (a) providing a unit of a substantially planar solid material having an attachment surface;
- (b) providing said species of proteins onto said attachment surface so that they are attached thereto;
- and
- (c) subdividing said unit of substantially planar material to form a plurality of separate tiles, a surface of each of said separate tiles comprising a portion of said attachment surface;
- and
- 2) affixing separate tiles from the said batches of tiles in predetermined spatial positions on a support.
A process for constructing a chemical array comprising a plurality of species of peptides in a predetermined arrangement, said process comprising the steps of:
- 1) for each species of said peptides, constructing a batch of separate tiles by:
- (a) providing a unit of a substantially planar solid material having an attachment surface;
- (b) providing said species of peptide onto said attachment surface so that they are attached thereto;
- and
- (c) subdividing said unit of substantially planar material to form a plurality of separate tiles, a surface of each of said separate tiles comprising a portion of said attachment surface;
- and
- 2) affixing separate tiles from the said batches of tiles in predetermined spatial positions on a support.
Dependencies are inferred from the claim text.