Methods of making non-transgenic herbicide resistant plants

A method for producing a non-transgenic, herbicide resistant or tolerant plant comprising:

• (a) introducing into plant cells a recombinagenic oligonucleobase with a targeted mutation in the EPSPS gene to produce plant cells with a mutant EPSPS gene that expresses a mutant EPSPS protein that is mutated at one or more amino acid positions, said positions selected from the group consisting of Leu173, Ala179, Met180, Arg181, Ser98, Ser255 and Leu198 in the Arabidopsis EPSPS protein or at an analogous amino acid residue in an EPSPS paralog;
• (b) identifying a plant cell having mutant EPSPS protein that has substantially the same catalytic activity as compared to a corresponding wild type EPSPS protein in the presence of glyphosate;
• and
• (c) regenerating a non-transgenic herbicide resistant or tolerant plant having a mutated EPSPS gene from said plant cell.

A method for producing a non-transgenic, herbicide resistant or tolerant plant comprising:

• (a) introducing into plant cells a recombinagenic oligonucleobase with a targeted mutation in the EPSPS gene to produce plant cells with a mutant EPSPS gene that expresses an EPSPS protein that is mutated in two amino acid positions, said positions selected from the group consisting of Thr178 and Pro182, in the Arabidopsis EPSPS protein or at an analogous amino acid residue in an EPSPS paralog wherein the Thr178 is changed to Val or Leu and Pro182 is changed to Ser;
• (b) identifying a plant cell having substantially normal growth as compared to a corresponding wild-type plant cell in the presence of glyphosate;
• and
• (c) regenerating a non-transgenic herbicide resistant or tolerant plant having a mutated EPSPS gene from said plant cell.