Protein purification
AssigneeGenentech, Inc.
InventorsTimothy N. Breece, Robert L. Fahrner, Jeffrey R. Gorrell, Kathlyn Pham Lazzareschi, Philip M. Lester, David Peng
Life of this patent
Estimating the term…
Abstract
A method for purifying proteins by Protein A chromatography is described which comprises removing contaminants by washing the solid phase with various intermediate wash buffers.
Claims19
A method for purifying a protein, which comprises a CH2/CH3 region, from a contaminated solution thereof by Protein A chromatography comprising:
- (a) adsorbing the protein from said contaminated solution to Protein A immobilized on a solid phase;
- (b) removing contaminants by washing the solid phase with a composition comprising detergent and salt at about pH 4.5 to about 5.5;
- and
- (c) recovering the protein from the solid phase with an elution buffer having a pH in the range from about 2 to about 5.
A method for purifying a protein, which comprises a CH2/CH3 region, from a contaminated solution thereof by Protein A chromatography comprising:
- (a) adsorbing the protein to Protein A immobilized on a solid phase;
- (b) removing contaminants by washing the solid phase with a composition comprising a buffer at a concentration of greater than about 0.8M;
- and
- (c) recovering the protein from the solid phase.
A method for purifying a protein, which comprises a CH2/CH3 region, from a contaminated solution thereof by Protein A chromatography comprising:
- (a) adsorbing the protein to Protein A immobilized on a solid phase;
- (b) removing contaminants by washing the solid phase with a composition comprising salt and a solvent selected from the group consisting of ethanol, methanol, isopropanol, acetonitrile, hexylene glycol, propylene glycol, and 2,2-thiodiglycol;
- and
- (c) recovering the protein from the solid phase.
A method for purifying a protein, which comprises a CH2/CH3 region, from a contaminated solution thereof by Protein A chromatography comprising:
- (a) adsorbing the protein to Protein A immobilized on a solid phase;
- (b) removing contaminants by washing the solid phase with a composition comprising salt and a polymer selected from the group consisting of polyethylene glycol, polypropyl glycol, and copolymers of ethylene and propylene glycol;
- and
- (c) recovering the protein from the solid phase.
Dependencies are inferred from the claim text.