DNA encoding novel D-aminoacylase and process for producing D-amino acid by using the same
AssigneeMitsui Chemicals, Inc.
InventorsMasami Osabe, Katsuyuki Takahashi, Toshifumi Yamaki, Teruo Arii, Toshihiro Oikawa
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Abstract
This invention provides a novel D-aminoacylase obtained by cloning a DNA encoding the novel D-aminoacylase from Methylobacterium mesophilicum MT 10894, etc. and which shows sufficiently high activity at an industrially useful substrate concentration to allow a D-amino acid to be efficiently produced from an N-acyl-DL-amino acid; a DNA encoding the D-aminoacylase; a process for producing a D-amino acid from the corresponding N-acylamino acid using a transformant containing the DNA.
Claims9
An isolated D-aminoacylase capable of catalyzing a reaction forming a D-amino acid by acting on the corresponding N-acyl-D-amino acid comprising the amino acid sequence of SEQ ID NO:
- 2.
A process for producing a D-amino acid comprising the step of forming said D-amino acid by the action of a D-aminoacylase on the corresponding N-acyl-D-amino acid, wherein said D-aminoacylase acts in an aqueous medium, and further wherein said D-aminoacylase comprises the amino acid sequence of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.