Method for synthesis, separation and screening of a plurality of compounds in the same bulk of a stationary phase
AssigneeSinvent AS
InventorLorenzo Williams
Life of this patent
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Abstract
A method for sequentially performing a synthesis, separation and screening of chemical entities, especially a combinatorial library, is described. The method utilises a bulk of a stationary phase (e.g. silica gel, aluminium oxide, cellulose, etc. for example arranged on a backing) for the performance of the synthesis, separation and screening. The technique described enables a rapid route from synthesis to the testing of chemical compounds. Screening can be performed without need for reaction work-up. Preferred screening methods are those used to determine the biological activity of the compounds.
Claims36
A method for preparing and screening a plurality of compounds, said method performed in or on a bulk of a stationary phase, the method comprises the sequential steps of
- (a) performing a synthesis of compounds by a chemical reaction performed in the bulk of a stationary phase,
- (b) separating the compounds in or on the same bulk of the stationary phase using a mobile phase;
- and
- (c) screening of the separated compounds in or on the same bulk of stationary phase,
- wherein said screening involves biological or biochemical methods;
- wherein the stationary phase is a thin-layer chromatography plate and wherein the stationary phase is suitable for sequential synthesis.
A method for preparing a plurality of compounds and screening at least one of said compounds, the method comprises the sequential steps of
- (a) performing a synthesis of compounds by a chemical reaction performed in or on the bulk of a stationary phase,
- (b) separating at least one of the compounds in or on the same bulk of the stationary phase using a mobile phase;
- and
- (c) screening the at least one separated compound in or on the same bulk of stationary phase, or via transferring or blotting of the at least one said compound from said bulk into or onto another medium;
- wherein said screening involves biological or biochemical methods;
- wherein the stationary phase is a part of a thin-layer chromatography plate or wherein the stationary phase is suitable for performing the method in a three dimensional bulk of the stationary phase.
Dependencies are inferred from the claim text.