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US 6,858,394 B1Granted
Composite arrays utilizing microspheres
AssigneeIllumina, Inc.
InventorsMark S. Chee, Steven R. Auger, John R. Stuelpnagel
Life of this patent
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Abstract
The invention relates to sensor compositions comprising a composite array of individual arrays, to allow for simultaneous processing of a number of samples. The invention further provides methods of making and using the composite arrays.
Claims14
A method of determining the presence of one or more target analytes in one or more samples comprising:
- a) adding said one or more samples to a first substrate comprising a plurality of assay locations, such that said one or more samples is contained at a plurality of said assay locations;
- b) contacting said one or more samples with a second substrate comprising:
- i) a plurality of array locations, each array location comprising a plurality of discrete sites, wherein at least one assay location is in fluid contact with at least one array location;
- and
- ii) a population of microspheres comprising at least a first and a second subpopulation each comprising a bioactive agent, wherein said microspheres are distributed on at least one of said array locations such that said discrete sites each contain no more than one microsphere;
- and
- c) determining the presence or absence of said target analyte.
4 more elements
A method of determining the presence of one or more target analytes in one or more samples comprising:
- a) adding said one or more samples to a first substrate comprising a plurality of assay locations, such that said one or more samples is contained at a plurality of said assay locations;
- b) contacting said one or more samples with a second substrate comprising:
- i) a composite array comprising a plurality of array locations, each array location comprising a plurality of discrete sites, wherein at least one assay location is in fluid contact with at least one array location;
- and
- ii) a population of microspheres comprising at least a first and a second subpopulation each comprising a bioactive agent, wherein said microspheres are distributed on said surface such that said discrete sites each contain no more than one microsphere;
- and
- c) determining the presence or absence of said target analyte.
4 more elements
Dependencies are inferred from the claim text.