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US 6,855,871 B2Granted
Methods of increasing polypeptide accumulation in plants
AssigneePioneer Hi-Bred International, Inc.
InventorsDarren B. Gruis, Rudolf Jung
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AssigneePioneer Hi-Bred International, Inc.
InventorsDarren B. Gruis, Rudolf Jung
Estimating the term…
The present invention provides methods and compositions directed to the production of polypeptides in plant protein storage tissue. Methods of the invention include methods for producing polypeptides in protein storage tissues by decreasing the level of protease activity in the plant protein storage tissue. Compositions of the invention include plants that are genetically modified such that the activity of one or more proteases is reduced or eliminated, transformed seeds obtained from these genetically modified plants, and sequences encoding a novel member of the VPE cysteine protease family found in seed, ε-VPE. These methods and compositions find utility in altering the nutritional or other qualities of seed, and in producing useful proteins including pharmacological or industrial proteins.
A plant that is genetically modified to reduce or eliminate the activity of one or more proteases in its protein storage tissue, wherein said protease is selected from the group consisting of α-vacuolar processing enzyme, β-vacuolar processing enzyme, γ-vacuolar processing enzyme, ε-vacuolar processing enzyme, aspartic protease AP1, and aspartic protease AP2, wherein said genetic modification is selected from the group consisting of insertional mutagenesis, antisense suppression and sense suppressions.
Dependencies are inferred from the claim text.