Nucleic acids encoding plant defensins and methods of use thereof
AssigneeE.I. du Pont de Nemours and Company
InventorsOmolayo O. Famodu, Rafael Herrmann, Albert L. Lu, Billy Fred McCutchen, Guo-Hua Miao, James K. Presnail, Zude Weng
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Abstract
This invention relates to isolated nucleic acids encoding plant defensins. The invention also relates to the construction of a chimeric gene encoding all or a portion of the plant defensin, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of plant defensins in a transformed host cell.
Claims41
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence having at least 80% sequence identity to SEQ ID NO:
- 7, SEQ ID NO:
- 29, or SEQ ID NO:
- 34 wherein said nucleotide sequence encodes a polypeptide having defensin activity against at least one pathogen;
- and
- (b) a nucleotide sequence that comprises the complement of (a).
A method for impacting resistance of a plant to at least one pathogen comprising introducing into a plant at least one DNA construct comprising a nucleotide sequence operably linked to a promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleotide sequence having at least 80% sequence identity to SEQ ID NO:
- 7, SEQ ID NO:
- 29, or SEQ ID NO:
- 34, wherein said nucleotide sequence encodes a polypeptide having defensin activity against at least one pathogen;
- and
- (b) a nucleotide sequence that comprises the complement of (a).
A method for enhancing resistance of a plant or cell thereof to the fungal pathogen Sclerotinia sclerotiorum comprising introducing into a plant or cell thereof at least one DNA construct comprising a nucleotide sequence operably linked to a promoter that drives expression in a plant cell, wherein said nucleotide sequence has at least 80% sequence identity to SEQ ID NO:
- 7, SEQ ID NO:
- 29, or SEQ ID NO:
- 34 and encodes a polypeptide having defensin activity against Sclerotinia sclerotiorum.
A method for enhancing resistance of a plant or cell thereof to at least one nematode comprising introducing into a plant or cell thereof at least one DNA construct comprising a nucleotide sequence operably linked to a promoter that drives expression in a plant cell, wherein said nucleotide sequence has at least 80% sequence identity to SEQ ID NO:
- 7, SEQ ID NO:
- 29, or SEQ ID NO:
- 34 and encodes a polypeptide having defensin activity against said nematode.
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence having at least 85% sequence identity to SEQ ID NO:
- 7, SEQ ID NO:
- 29, or SEQ ID NO:
- 34 wherein said nucleotide sequence encodes a polypeptide having defensin activity against at least one pathogen;
- and
- (b) a nucleotide sequence that comprises the complement of (a).
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence having at least 90% sequence identity to SEQ ID NO:
- 7, SEQ ID NO:
- 29, or SEQ ID NO:
- 34 wherein said nucleotide sequence encodes a polypeptide having defensin activity against at least one pathogen;
- and
- (b) a nucleotide sequence that comprises the complement of (a).
A method for impacting resistance of a plant to at least one pathogen comprising introducing into a plant at least one DNA construct comprising a nucleotide sequence operably linked to a promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleotide sequence having at least 85% sequence identity to SEQ ID NO:
- 7, SEQ ID NO:
- 29, or SEQ ID NO:
- 34, wherein said nucleotide sequence encodes a polypeptide having defensin activity against at least one pathogen;
- and
- (b) a nucleotide sequence that comprises the complement of (a).
A method for impacting resistance of a plant to at least one pathogen comprising introducing into a plant at least one DNA construct comprising a nucleotide sequence operably linked to a promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleotide sequence having at least 90% sequence identity to SEQ ID NO:
- 7, SEQ ID NO:
- 29, or SEQ ID NO:
- 34, wherein said nucleotide sequence encodes a polypeptide having defensin activity against at least one pathogen;
- and
- (b) a nucleotide sequence that comprises the complement of (a).
Dependencies are inferred from the claim text.