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US 6,855,814 B2Granted
Sequences of E. coli O157
AssigneeWisconsin Alumni Research Foundation
InventorsFrederick R. Blattner, Valerie Burland, Nicole T. Perna, Guy Plunkett, Rod Welch
Life of this patent
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Abstract
The entire genome of pathogenic E. coli strain 0157:H7 has been sequenced. All of the genomic DNA sequences present in 0157 and absent in the previously sequenced laboratory strain K12 are presented here.
Claims4
An isolated DNA molecule comprising the nucleotide sequence of SEQ ID NO:256.
A method for detecting Escherichia coli O157:H7 (ATCC 43895) in a sample comprising the steps of:
- a) providing an E. coli O157:H7-specific nucleotide probe, the probe comprising a sequence as set forth in SEQ ID NO:256;
- b) contacting the probe with a sample of test material under suitable hybridization conditions;
- and
- c) detecting hybridization of the probe to a complementary nucleotide sequence in the sample.
1 more element
Dependencies are inferred from the claim text.