Pharmaceutical compositions of conjugated estrogens and methods of analyzing mixtures containing estrogenic compounds

A composition of matter comprising:

• a mixture of estrogenic compounds, wherein at least one of said estrogenic compounds is a synthetic estrogenic compound, and wherein said mixture comprises the same essential estrogenic compounds present in naturally derived equine conjugated estrogens;
• wherein said composition of matter is present in a chemically pure form.

The composition according to claim wherein the mixture further comprises at least one compound selected from the group consisting of equilenin, 17β-dihydroequilenin, 17β-dihydroequilin, 17α-dihydroequilenin, 17β-estradiol, 17α-dihydroequilin, 17α-estradiol, equilin, Δ8,9-dehydroestrone, estrone, 17α-Δ8,9-dehydroestradiol, 17β-Δ8,9-dehydroestradiol, 6-OH 17α-dihydroequilenin, 6-OH equilenin, and the salts of 17αΔ8,9-dehydroestradiol sulfate, 17β-Δ8,9-dehydroestradiol sulfate, 6-OH 17α-dihydroequilenin sulfate, 6-OH 17β-dihydroequilenin sulfate, and 6-OH equilenin sulfate.

A composition of matter comprising:

• a mixture of estrogenic compounds, wherein said mixture comprises the same essential estrogenic compounds present in naturally derived equine conjugated estrogens;
• wherein said composition of matter is present in a chemically pure form.

A composition of matter comprising:

• a mixture of estrogenic compounds, wherein said mixture comprises salts of conjugated estrone, conjugated equilin, conjugated Δ8,9-dehydroestrone, conjugated 17α-estradiol, conjugated 17α-dihydroequilin, conjugated 17β-dihydroequilin, conjugated 17β-estradiol, conjugated equilenin, conjugated 17α-dihydroequilenin, and conjugated 17β-dihydroequilenin, and wherein said mixture comprises the same essential estrogenic compounds present in naturally derived equine conjugated estrogens;
• wherein said composition of matter is substantially devoid of a compound selected from the group consisting of indican, sulfated benzyl alcohol, hippuric acid, benzoic acid, and creatinine.

A composition of matter comprising:

• a mixture of estrogenic compounds, wherein said mixture comprises salts of conjugated estrone, conjugated equilin, conjugated Δ8,9-dehydroestrone, conjugated 17α-estradiol, conjugated 17α-dihydroequilin, conjugated 17β-dihydroequilin, conjugated 17β-estradiol, conjugated equilenin, conjugated 17α-dihydroequilenin, and conjugated 17β-dihydroequilenin, and wherein said mixture comprises the same essential estrogenic compounds present in naturally derived equine conjugated estrogens;
• wherein said composition of matter is substantially devoid of indican, sulfated benzyl alcohol, hippuric acid, benzoic acid, and creatinine.