Recombinant protein production in a human cell
AssigneeCrucell Holland B.V.
InventorsGuus Hateboer, Karina Cornelia Verhulst, Govert Johan Schouten, Alphonsus Gerardus Uytdehaag, Abraham Bout
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Abstract
Methods and compositions for the production of recombinant proteins in a human cell line. The methods and positions are particularly useful for generating stable expression of human recombinant proteins of interest that are modified post-translationally, for example, by glycosylation. Such proteins may have advantageous properties in comparison with their counterparts produced in non-human systems such as Chinese Hamster Ovary cells.
Claims28
A method for producing at least one proteinaceous substance in a eukaryotic cell, said method comprising:
- providing a eukaryotic cell having a nucleic acid sequence in the eukaryotic cell's genome, said nucleic acid sequence encoding adenoviral E1A and E1B proteins, which eukaryotic cell further does not comprise a sequence encoding a structural adenoviral protein in its genome;
- introducing a gene encoding a recombinant proteinaceous substance into the eukaryotic cell;
- culturing said eukaryotic cell in a suitable medium;
- and
- harvesting at least one proteinaceous substance from said eukaryotic cell, said suitable medium, or both said eukaryotic cell and said medium.
A method for producing at least one human recombinant protein in a cell, said method comprising:
- providing a human cell with a gene encoding a human recombinant protein, wherein said human cell has in its genome a sequence encoding adenoviral E1A and E1B proteins, wherein said human cell further does not produce structural adenoviral proteins and does not comprise a sequence encoding a structural adenoviral protein in its genome;
- culturing said human cell in a suitable medium;
- and
- harvesting the human recombinant protein from the human cell, the suitable medium, or both said human cell and said medium.
Dependencies are inferred from the claim text.