Materials and methods for the enhancement of effective root nodulation in legumes
AssigneeThe University of Tennessee Research Corporation
InventorsJohn T. Loh, Gary Stacey
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Abstract
The subject invention relates to compounds and compositions which induce transcripts of the nolA gene in nitrogen-fixing bacteria, such as Bradyrhizobium japonicum. Novel bacterial strains which are insensitive too NolA, soil inoculants comprising NolA insensitive bacteria and/or nolA inducers, and methods of increasing nitrogen fixation in legumes are also disclosed.
Claims9
A method of more efficiently nodulating a target plant species comprising producing a nodulation inoculant comprising culturing at least one strain of Bradyrhizobium japonicum in a liquid medium wherein the medium comprises a sufficient amount of iron to reduce the amount of Cell Density Factor (CDF) expression and then applying said inoculant to a target plant, parts of a target plant, or seeds thereof.
A method of more efficiently nodulating a target plant species comprising the steps of:
- a) producing a nodulation inoculant by culturing at least one strain of Bradyrhizobium japonicum in a liquid medium wherein the medium comprises a sufficient amount of iron to reduce the amount of Cell Density Factor (CDF) expression;
- b) incorporating said nodulation inoculant into soil;
- and
- c) and then applying seed or plant material of said target plant to said soil.
A method of improving the efficiency of soil inoculation comprising producing a nodulation inoculant by culturing at least one strain of Bradyrhizobium japonicum in a liquid medium wherein the medium comprises a sufficient amount of iron to reduce the amount of Cell Density Factor (CDF) expression, applying said inoculant soil, and growing plants in said soil.
Dependencies are inferred from the claim text.