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US 6,855,535 B2Granted
Method of large scale production of Hepatitis A virus
AssigneeBaxter Healthcare S.A.
InventorsHeidi Meyer, Manfred Reiter, Wolfgang Mundt, Noel Barrett, Friedrich Dorner
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Abstract
The present invention provides methods of large scale production of Hepatitis A Virus (HAV) on VERO cells bound to microcarrier. The invention also provides for methods of isolation of HAV from the cell culture supernatant of HAV infected VERO cells.
Claims13
A method for continuous production of Hepatitis A virus (HAV) antigen, comprising the steps of providing a serum free cell culture of VERO cells bound to a microcarrier;
- growing the VERO cells in said serum free cell culture;
- infecting said serum free cell culture of VERO cells with HAV at a reduced temperature compared to the step of growing the cells;
- incubating said serum free cell culture of VERO cells infected with HAV to propagate said HAV at the reduced temperature, whereby HAV antigen is continuously released into the cell culture medium because infected cells release at least 50% of viral antigen into said medium;
- and harvesting said HAV antigen released into said medium.
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Dependencies are inferred from the claim text.