Gene conferring lysozyme insensitivity to corynebacterium
AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsKazuo Nagai, Masaaki Wati
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Abstract
The present invention relates to a protein having an activity of giving a lysozyme insensitivity to a lysozyme-sensitive microorganism belonging to Corynebacterium glutamicum; DNA which codes for the protein; a recombinant vector containing the DNA; a transformant obtained by introducing the recombinant vector into a host cell; a bacterium having a lysozyme sensitivity in which the activity of the protein is inactivated; and a method for producing an amino acid using the bacterium.
Claims25
An isolated DNA encoding a protein which comprises the amino acid sequence of SEQ ID NO:
- 2, and confers to a Corynebacterium glutamicum microorganism an ability to grow in a medium containing 1% polypeptone, 0.5% yeast extract, 0.5% sodium chloride, 0.1% glucose, 20 μg/ml thiamine and 100 μg/ml lysozyme.
An isolated DNA comprising the nucleotide sequence of SEQ ID NO:
- 1;
- or a DNA hybridizing with the DNA having a complementary nucleotide sequence of SEQ ID NO:
- 1 at 65 ° C. in the presence of 0.7 to 1.0 M sodium chloride and encoding a protein which confers to a Corynebacterium glutamicum microorganism an ability to grow in a medium containing 1% polypeptone, 0.5% yeast extract, 0.5% sodium chloride, 0.1% glucose, 20 μg/ml thiamine and 100 μg/ml lysozyme, wherein the hybridization further includes a step of washing under the condition of 65° C. by the use of solution containing 15 to 300 mM sodium chloride and 1.5 to 30 mM sodium citrate.
An isolated DNA which is contained in a plasmid carried by FERM BP-6479 and codes for a protein which confers to a Corynebacterium glutamicum microorganism an ability to grow in a medium containing 1% polypeptone, 0.5% yeast extract, 0.5% sodium chloride, 0.1% glucose, 20 μg/ml thiamine and 100 μg/ml lysozyme.
A DNA fragment which comprises a nucleotide sequence of the nucleotide position numbers 271 to 1593 in the nucleotide sequence identified as SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.