Method for detecting presence of microorganisms, and quantities of microorganisms

A method for identifying quantities of microorganisms in a sample, comprising the steps of:

• (a) preparing a container comprising a medium portion which has a fluid culture medium for supporting growth of microorganisms and a fluid indicator isolated from the medium portion which contains a color changing CO2 indicator for detecting the presence of microorganisms;
• (b) wherein said fluid indicator portion is isolated from said medium portion by a CO2 gas-permeable membrane, which permeates CO2 gas at a pressure required to maintain a density of CO2 gas at a level required for growth of a microorganism;
• (c) mixing a sample in said fluid culture medium;
• (d) sealing said container entirely from outside atmosphere;
• (e) measuring time, starting from a moment when said container is sealed until a moment when color of said CO2 indicator is turned into a predetermined color;
• and
• (f) identifying initial quantities of microorganisms obtained by comparing measured time against contents of a table which holds pre-collected time data on each microorganism species of known initial quantities in known amount of sample.

A method for identifying quantities of microorganisms in a sample, comprising the steps of:

• (a) preparing a container comprising a medium portion which has a fluid culture medium for supporting growth of microorganisms and an indicator isolated from the medium portion which contains a color changing CO2 indicator for detecting the presence of microorganisms;
• (b) wherein said indicator portion is isolated from said medium portion by a CO2 gas-permeable membrane, which permeates CO2 gas at a pressure required to maintain a density of CO2 gas at a level required for growth of a microorganism;
• (c) mixing a sample in said fluid culture medium;
• (d) sealing said container entirely from outside atmosphere;
• (e) measuring time, starting from a moment when said container is sealed until a moment when color of said CO2 indicator is turned into a predetermined color;
• and
• (f) identifying initial quantities of microorganisms obtained by comparing measured time against contents of a table which holds pre-collected time data on each microorganism species of known initial quantities in known amount of sample.