Quorum sensing signaling in bacteria
AssigneesUniversity of Iowa Research Foundation, Vertex Pharmaceuticals (San Diego) LLC
InventorsMarvin Whiteley, Kimberly M. Lee, E. Peter Greenberg, Ute Muh
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Abstract
The invention provides methods for identifying a modulator of quorum sensing signaling in bacteria, and for identifying a quorum sensing controlled gene in bacteria. In addition, the invention provides quorum sensing controlled genetic loci in Pseudomas aeruginosa. Novel indicator strains and vectors for engineering the strains for use in the method of the invention are also provided.
Claims33
A method for identifying a modulator of quorum sensing signaling in bacteria, said method comprising:
- providing a cell which is capable of endogenously synthesizing a quorum sensing signal molecule, wherein said cell comprises a regulatory sequence of a quorum sensing controlled gene operatively linked to a gene that generates a detectable signal in response to the quorum sensing signal molecule;
- contacting said cell with a test compound, wherein said test compound is other than said quorum sensing signal molecule;
- and comparing said detectable signal generated in the presence of said test compound with said detectable signal generated in the absence of said test compound, to thereby identify said test compound as said modulator of quorum sensing signaling in bacteria.
A method for identifying a modulator of quorum sensing signaling in bacteria, said method comprising:
- providing a cell which comprises a quorum sensing controlled gene wherein said quorum sensing controlled gene comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35 and SEQ ID NO:36, operatively linked to a gene that generates a detectable signal in response to a quorum sensing signal molecule;
- contacting said cell with said quorum sensing signal molecule in the presence and absence of a test compound;
- and comparing said detectable signal generated in the presence of said test compound with said detectable signal generated in the absence of said test compound, to thereby identify said test compound as said modulator of quorum sensing signaling in bacteria.
A method for identifying a modulator of quorum sensing signaling in bacteria, said method comprising:
- providing a cell which comprises a regulatory sequence of a quorum sensing controlled gene operatively linked to a gene that generates a detectable signal in response to a quorum sensing signal molecule;
- contacting said cell with 3-oxo-C12 homoserine lactone in the presence and absence of a test compound;
- and comparing said detectable signal generated in the presence of said test compound with said detectable signal generated in the absence of said test compound, to thereby identify said test compound as said modulator of quorum sensing signaling in bacteria.
A method for identifing a modulator of quorum sensing signaling in bacteria, said method comprising:
- providing a cell which comprises a regulatory sequence of a quorum sensing controlled gene operatively linked to a gene that generates a detectable signal in response to a quorum sensing signal molecule;
- contacting said cell with said quorum sensing signal molecule in the presence and absence of a test compound;
- and detecting a change in said detectable signal to thereby identify said test compound as a modulator of quorum sensing signaling in bacteria.
Dependencies are inferred from the claim text.