Protein S functional assay and kit therefor
AssigneeInstrumentation Laboratory Company
InventorsYong Dai, Biqing Ye, Kui Chen, Pau Bruguera, Daniel E. Lawson, ShaMay Tang
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Abstract
The invention relates generally to a new functional protein S assay and kit that is based on the ability of endogenous protein S to prolong clotting time. In the assay procedure, a test plasma sample is diluted with protein S deficient plasma, followed by the addition of purified or recombinant tissue factor (pTF or rTF), purified natural or synthetic phospholipid (pPL or sPL) and activated protein C (APC) or protein C activator (PCA). The clotting time is then measured and compared to a standard curve or a normal control.
Claims26
A method for measuring protein S activity in a test plasma sample comprising:
- (a) mixing a sample of test plasma with protein S deficient plasma,
- at least one recombinant tissue factor selected from the group consisting of recombinant rabbit, recombinant porcine, recombinant equine and recombinant human tissue factors,
- purified or synthetic phospholipids comprising phosphocholine, phosphoserine and phosphoethanolamine,
- activated protein C,
- calcium ion,
- and measuring a clotting time of the sample,
- (b) comparing the measurement in (a) to a standard curve derived from clotting times of plasma samples having a range of known protein S activities.
A kit for measuring the functional activity of protein S in a plasma sample, said kit comprising one or more containers containing
- protein S deficient plasma,
- at least one recombinant tissue factor selected from the group consisting of recombinant rabbit, recombinant porcine, recombinant equine and recombinant human tissue factors,
- purified or synthetic phospholipids comprising phosphocholine, phosphoserine and phosphoethanolamine,
- calcium ion and
- activated protein C.
Dependencies are inferred from the claim text.