ELISA for VEGF
AssigneeGenentech, Inc.
InventorsDavid Tai Wai Fei, Kristen Tomita
Life of this patent
Estimating the term…
Abstract
The vascular endothelial growth factor (VEGF) activity in a patient's bloodstream or other biological sample can serve as a diagnostic and prognostic index for cancer, diabetes, heart conditions, and other pathologies. Antibody-sandwich ELISA method and kits for VEGF as an antigen were developed to detect VEGF levels in biological samples from animal models and human patients and are used as a diagnostic/prognostic index.
Claims31
A method for detecting multiple isoforms of vascular endothelial growth factor (VEGF) in a biological sample, comprising:
- (a) incubating a biological sample with a capture reagent immobilized on a solid support to bind multiple isoforms of VEGF to the capture reagent, wherein the capture reagent comprises a mixture comprising a polyclonal antibody that binds VEGF and a monoclonal antibody that specifically binds to amino acid residues 111-165 of human VEGF;
- and
- (b) detecting VEGF bound to the immobilized capture reagent by contacting the bound VEGF with a detectable antibody that binds to N-terminal amino acid residues 1-110 of VEGF.
An immunoassay kit for detecting multiple isoforms of vascular endothelial growth factor (VEGF) in a biological sample, the kit comprising:
- (a) as a capture reagent, a mixture comprising a polyclonal antibody that binds VEGF and a monoclonal antibody that specifically binds to amino acid residues 111-165 of human VEGF;
- and
- (b) as a detection reagent, a detectable antibody that binds to N-terminal amino acid residues 1-110 of VEGF.
Dependencies are inferred from the claim text.