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US 6,855,505 B2Granted
Method for quantifying TGF-β
AssigneeRenovo Limited
InventorsMark W. J. Ferguson, Georg Brunner
Life of this patent
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Abstract
The present invention relates to a method for quantifying the amount of active transforming growth factor beta (TGF-β) in biological samples. In particular the method comprising incubating cryosections of a tissue with cells which contain a TGF-β responsive expression vector having a structural region encoding an indicator protein and measuring the amount of said indicator protein expressed from the cells.
Claims18
An assay for quantifying the amount of active transforming growth factor β (TGF-β) in a sample, the TGF-β assay comprising:
- (a) incubating the sample together with eukaryotic cells which contain a TGF-β responsive expression vector having a structural region encoding an indicator protein for a predetermined time period sufficient for said eukaryotic cells to express a detectable amount of said indicator protein;
- (b) measuring the amount of said indicator protein expressed during said time period;
- and
- (c) determining the amount of TGF-β present in the sample by comparing the measured amount of said indicator protein against reference data;
- wherein the sample comprises a cryosection of a tissue, said cryosection having been treated such that said TGF-β is in an active and/or latent form.
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