In vivo high throughput toxicology screening method
AssigneeTosk, Inc.
InventorPatrick Fogarty
Life of this patent
Estimating the term…
Abstract
A high throughput toxicology screening method is provided. In the subject method, at least 10 different compound compositions are tested simultaneously. Each compound composition is tested by contacting it with a plurality, e.g. from about 10 to 1000, of non-mammalian multi-cellular organisms and determining the effect of the compound composition on the organisms. The multi-cellular organisms employed in the subject methods are small, have differentiated tissues and organs and have a rapid generation time. The subject high throughput screening methods find use in a variety of applications, and are particularly suited for use in the toxicology screening of libraries of compounds, such as libraries of combinatorially produced compounds.
Claims16
A high throughput toxicology screening method in which at least 10 different compound compositions are simultaneously assayed for toxicity, said method comprising:
- simultaneously assaying at least 10 different compound compositions for toxicity, wherein each of said at least 10 different compound compositions is assayed for toxicity by:
- (a) contacting said compound composition with a plurality of non-mammalian multi-cellular organisms;
- and
- (b) determining the effect of said compound composition on said non-mammalian multi-cellular organisms to estimate the effect of said compound composition on a mammalian organism selected from a rat or a mouse.
A high throughput toxicology screening method in which at least 10 different compound compositions are simultaneously assayed for toxicity, said method comprising:
- simultaneously assaying at least 10 different compound compositions for toxicity, wherein each of said at least 10 different compound compositions is assayed or toxicity by:
- (a) contacting said compound composition with a population of from about 10 to 1000 small non-mammalian multi-cellular organisms having a rapid generation time and differentiated organs end tissues;
- and
- (b) determining the effect of said compound composition on said non-mammalian multi-cellular organisms to estimate the effect of said compound composition on a mammalian organism selected from a rat or a mouse.
A high throughput antitoxin screening method in which at least 10 different candidate antitoxin compound compositions are simultaneously assayed for antitoxin activity, said method comprising:
- simultaneously assaying at least 10 different candidate antitoxin compound compositions for antitoxin activity, wherein each of said at least 10 different candidate compound compositions is assayed for antitoxin activity by:
- (a) contacting said candidate compound composition with a population of from about 10 to 1000 small non-mammalian multi-cellular organisms having a rapid generation time and differentiated organs and tissues which have been contacted with a toxin;
- and
- (b) determining the effect of said compound composition on said non-mammalian multi-cellular organisms to estimate the effect of said compound composition on a mammalian organism selected from a rat or a mouse.
Dependencies are inferred from the claim text.