Transfer of arrayed chemical compositions
AssigneeLinden Technologies, Inc.
InventorTai-nang Huang
Life of this patent
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Abstract
Nucleotide preparations are transferred from a first substrate to a second substrate. One transfer method includes forming a patterned dry particulate deposition on a first substrate; positioning the first substrate in apposition to a second substrate; and transferring at least a portion of the dry deposition from the first substrate to the second substrate to produce a patterned dry deposition of the nucleotide on the second substrate. The method can be used to form an array of nucleic acids.
Claims26
A method of providing a nucleic acid array comprising:
- forming a patterned dry deposition on a first substrate, the dry deposition comprising a nucleotide in monomeric form;
- positioning the first substrate in apposition to a second substrate;
- and
- transferring at least a portion of the dry deposition from the first substrate to the second substrate to produce a patterned dry deposition of the nucleotide on the second substrate.
A method of providing a nucleic acid array comprising:
- forming a dry deposition on a first substrate, the dry deposition comprising a nucleotide;
- positioning the first substrate in apposition to a second substrate;
- and
- transferring at least a portion of the dry deposition from the first substrate to the second substrate by applying an electrical field generated by a field generator that comprises a corotron or scorotron, to produce a dry deposition of the nucleotide on the second substrate.
A method of providing a nucleic acid array comprising:
- forming a patterned dry deposition on a first substrate, the dry deposition comprising triboelectrically chargeable particles that include a nucleotide that includes a protecting group and a phosphoramidite, the nucleotide being in monomeric form, wherein the particles are less than 30 μm in diameter and the first substrate is flexible;
- positioning the first substrate in apposition to a second substrate;
- and
- transferring at least a portion of the dry deposition from the first substrate to the second substrate to produce a patterned dry deposition of the nucleotide on the second substrate.
A method of providing a nucleic acid array, the method comprising:
- providing a target substrate;
- providing a plurality of interim substrates, each interim substrate comprising different particles of a nucleic acid subunit that comprises a protecting group and an activated group, the particles being disposed at different addresses of the interim substrate;
- and
- sequentially, for each interim substrate of the plurality of interim substrates, transferring the particles from the different addresses of the interim substrate to corresponding addresses on the target substrate, wherein the transferring comprises scanning an electric field generator across an interim substrate, and coupling the subunit to the target substrate to form a nucleic acid at each of the different addresses of the target substrate.
A method of providing a nucleic acid array comprising:
- forming a dry deposition on a first substrate, the dry deposition comprising a nucleic acid polymer component;
- positioning the first substrate in apposition to a second substrate;
- and
- scanning an electrical field generator across the first substrate, the generator applying an electric field, thereby transferring at least a portion of the dry deposition from the first substrate to the second substrate.
Dependencies are inferred from the claim text.