Magnetic isolation and purification of nucleic acids
AssigneeCortex Biochem, Inc.
InventorRuhangiz D. Nargessi
Life of this patent
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Abstract
A method for the isolation and purification of nucleic acids such as DNA, RNA, and PNA from various sources using magnetizable cellulose or its derivatives. Adjusting the concentrations of the salt and polyalkylene glycol to the levels that result in binding of nucleic acids to the magnetizable cellulose or its derivatives. Separating the nucleic acids bound to the magnetizable cellulose particles or its derivatives and eluting the nucleic acids from the particles.
Claims31
A method to bind nucleic acids to magnetizable cellulose comprising
- a) combining magnetizable cellulose with a solution containing nucleic acids, thereby producing a combination, and
- b) adjusting the salt and polyalkylene glycol concentrations of the combination to concentrations suitable for binding the nucleic acids to the magnetizable cellulose, whereby all or a portion of the nucleic acids in the solution binds to the magnetizable cellulose.
A method of separating nucleic acids front non-nucleic acid materials in a nucleic acid solution, comprising:
- a) combining magnetizable cellulose with a solution containing nucleic acids and non-nucleic acid materials to produce a first combination;
- b) adjusting the salt and polyethylene glycol concentrations of the first combination to concentrations suitable for binding nucleic acids in the solution to the magnetizable cellulose, producing a second combination comprising magnetizable cellulose-bound nucleic acids;
- c) separating the magnetizable cellulose-bound nucleic acids from the second combination;
- d) contacting the magentizable cellulose-bound nucleic acids separated in c) with an elution buffer to release the bound nucleic acids from the magnetizable cellulose and into the elution buffer;
- and
- e) separating the magnetizable cellulose from the elution buffer to provide nucleic acids that are substantially free of the non-nucleic acid materials.
A method to bind nucleic acids to magnetizable cellulose derivatives, comprising:
- a) combining magnetizable cellulose derivatives with a solution containing nucleic acids, thereby producing a combination, and
- b) adjusting the salt and polyalkylene glycol concentrations of the combination to concentrations suitable for binding the nucleic acids to the magnetizable cellulose derivatives, whereby all or a portion of the nucleic acids in the solution bind to the magnetizable cellulose derivatives.
A method of separating nucleic acids from non-nucleic acid materials, comprising:
- a) combining magnetizable cellulose derivatives with a solution containing nucleic acids and non-nucleic acid materials to provide a first combination;
- b) adjusting the salt and polyethylene glycol concentrations of the first combination to concentrations suitable for binding nucleic acids to the magnetizable cellulose derivatives, producing a second combination comprising magnetizable cellulose derivative-bound nucleic acids;
- c) separating the magnetizable cellulose derivative-bound nucleic acids from the second combination;
- d) contacting the magnetizable cellulose derivative-bound nucleic acids separated in c) with an elution buffer to release the bound nucleic acids from the magnetizable cellulose derivatives and into the elution buffer;
- and
- e) separating the magnetizable cellulose derivatives from the elution buffer to provide nucleic acids that are substantially free of the non-nucleic acid materials.
Dependencies are inferred from the claim text.