Method for attaching biological molecules to a glass surface
AssigneeMedical Discovery Partners LLC
InventorsSeshi R. Sompuram, Halasya Ramanathan
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Abstract
The invention describes a method for attaching biological molecules, such as proteins, nucleic acids, and carbohydrates to a glass surface. In particular, a covalent attachment method is described wherein a glass surface is first activated with a water-stable form of an isocyanate, termed a protected isocyanate. The protecting group of the isocyanate moiety is displaced by amines, hydroxyl, or carboxyl groups of biological molecules, leading to covalent attachment to the glass surface.
Claims16
A method for attaching a biological molecule to a glass surface comprising the steps of:
- a) providing a silane-treated glass surface having an end-capped amino group;
- b) reacting said end-capped amino group with a phosgene equivalent to form a protected isocyanate group;
- and
- c) reacting said protected isocyanate group with a biological molecule,
- thereby forming a covalent coupling and attaching said biological molecule to the glass surface.
A method for attaching a biological molecule to a glass surface comprising the steps of:
- a) providing a silane-treated glass surface having an end-capped amino group;
- b) reacting said amino group with a phosgene equivalent to form an end-capped group, said end-capped group including a functional group represented by the following structural formula:
- >N—C(O)—N<
- or by the following structural formula:
- >N—C(O)—O—N<;
- and
- c) reacting said end-capped group with a biological molecule, thereby forming a covalent coupling and attaching said biological molecule to the glass surface.
A method for attaching a biological molecule to a silane-treated glass surface comprising the steps of:
- a) reacting an end-capped amino group on said silane-treated glass surface with a phosgene equivalent to form a protected isocyanate group;
- and
- b) reacting said protected isocyanate group with a biological molecule,
- thereby forming a covalent coupling and attaching said biological molecule to said silane-treated glass surface.
A method for attaching a biological molecule to a silane-treated glass surface comprising the steps of:
- a) reacting an amino group on said silane-treated glass surface with a phosgene equivalent to form an end-capped group, said end-capped group including a functional group represented by the following structural formula:
- >N—C(O)—N<
- or by the following structural formula:
- >N—C(O)—O—N<;
- and
- b) reacting said end-capped group with a biological molecule, thereby forming a covalent coupling and attaching said biological molecule to said silane-treated glass surface.
Dependencies are inferred from the claim text.