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US 6,855,292 B2Granted
In situ heat induced antigen recovery and staining apparatus and method
OwnerLee Angrosinventor
Life of this patent
Estimating the term…
Abstract
An automated in situ heat induced antigen recovery and staining method and apparatus for treating a plurality of microscope slides. The process of heat induced antigen recovery and the process of staining the biological sample on the microscope slide are conducted in the same apparatus, wherein the microscope slides do not need to by physically removed from one apparatus to another. Each treatment step occurs within the same reaction compartment. The reaction conditions of each reaction compartment for treating a slide can preferably be controlled independently, including the individualized application of reagents to each slide and the individualized treatment of each slide. The reagents are preferably held in a reagent dispensing strip similar to a “blister pack”.
Claims97
An in situ antigen recovery and staining apparatus for treating biological samples on microscope slides, comprising:
- a slide support assembly comprising a plurality of slide support elements, each slide support element able to support a microscope slide;
- a heating element incorporated into or adjacent each slide support element for heating a microscope slide on the slide support element;
- and
- a treatment chamber comprising a plurality of closeable reaction compartments, each reaction compartment having an openable bottom comprising a portion of the slide support element and an upper side having an upper opening through which a reagent can be delivered into an inner space of the reaction compartment.
1 more element
An in situ antigen recovery and staining apparatus for treating biological samples on microscope slides, comprising:
- a slide support assembly comprising a plurality of slide support elements, each slide support element able to support a microscope slide;
- a heating element incorporated into or adjacent each slide support element for heating a microscope slide on the slide support element;
- and
- a treatment chamber comprising a plurality of closeable reaction compartments, each reaction compartment having an openable bottom comprising a portion of the slide support element and an upper side having an upper opening through which reagents can be delivered into an inner space of the reaction compartment, wherein the reagents can be delivered into the plurality of reaction compartments simultaneously.
1 more element
An in situ antigen recovery and staining apparatus for treating biological samples on microscope slides, comprising:
- a slide support assembly comprising a plurality of slide support elements, each slide support element able to support a microscope slide;
- a heating element incorporated into or adjacent each slide support element for heating a microscope slide on the slide support element;
- and
- a treatment chamber comprising a plurality of closeable reaction compartments, each reaction compartment having an openable bottom comprising a portion of the slide support element and an upper side having an upper opening through which a reagent can be delivered into an inner space of the reaction compartment and wherein the heating element can heat the reagent to a temperature of from about 90° C. to about 100° C.
1 more element
An in situ antigen recovery and staining apparatus for treating biological samples on microscope slides, comprising:
- a slide support assembly comprising a plurality of slide support elements, each slide support element able to support a microscope slide;
- a heating element incorporated into or adjacent each slide support element for heating a microscope slide on the slide support element wherein the heating element can operate to independently heat each microscope slide disposed on the slide support assembly;
- and
- a treatment chamber comprising a plurality of closeable reaction compartments, each reaction compartment having an openable bottom comprising a portion of the slide support element and an upper side having an upper opening through which a reagent can be delivered into an inner space of the reaction compartment.
1 more element
An in situ antigen recovery and staining apparatus for treating biological samples on microscope slides, comprising:
- a slide support assembly comprising a plurality of slide support elements, each slide support element able to support a microscope slide wherein each slide support element is independently movable;
- a heating element incorporated into or adjacent each slide support element for heating a microscope slide on the slide support element;
- and
- a treatment chamber comprising a plurality of closeable reaction compartments, each reaction compartment having an openable bottom comprising a portion of the slide support element and an upper side having an upper opening through which a reagent can be delivered into an inner space of the reaction compartment.
1 more element
An in situ antigen recovery and staining apparatus for treating biological samples on microscope slides, comprising:
- a slide support assembly comprising a plurality of slide support elements, each slide support element able to support a microscope slide, wherein each slide support element is independently movable;
- a heating element incorporated into or adjacent each slide support element for heating a microscope slide on the slide support element wherein the heating element can operate to independently heat each microscope slide disposed on the slide support element;
- and
- a treatment chamber comprising a plurality of closeable reaction compartments, each reaction compartment having an openable bottom comprising a portion of the slide support element and an upper side having an upper opening through which a reagent can be delivered into an inner space of the reaction compartment.
1 more element
An in situ antigen recovery and staining apparatus for treating biological samples on microscope slides, comprising:
- a slide support assembly comprising a plurality of slide support elements, each slide support element able to support a microscope slide;
- a heating element incorporated into or adjacent each slide support element for heating a microscope slide on the slide support element;
- a treatment chamber comprising a plurality of closeable reaction compartments, each reaction compartment having an openable bottom comprising a portion of the slide support element and an upper side having an upper opening;
- and
- a reagent dispenser for dispensing a reagent onto the microscope slide supported on the slide support element.
2 more elements
An in situ antigen recovery and staining apparatus for treating biological samples on microscope slides, comprising:
- a slide support assembly comprising a plurality of slide support elements, each slide support element able to support a microscope slide;
- a heating element incorporated into or adjacent each slide support element for heating a microscope slide on the slide support element;
- a treatment chamber comprising a plurality of closeable reaction compartments, each reaction compartment having an openable bottom comprising a portion of the slide support element and an upper side having an upper opening;
- and
- a reagent dispenser for dispensing reagents onto the microscope slide supported on the slide support element, and wherein the reagents can be delivered onto the microscope slides simultaneously.
2 more elements
An in situ antigen recovery and staining apparatus for treating biological samples on microscope slides, comprising:
- a slide support assembly comprising a plurality of slide support elements, each slide support element able to support a microscope slide;
- a heating element incorporated into or adjacent each slide support element for heating a microscope slide on the slide support element;
- a treatment chamber comprising a plurality of closeable reaction compartments, each reaction compartment having an openable bottom comprising a portion of the slide support element and an upper side having an upper opening;
- and
- a reagent dispenser for dispensing a reagent onto the microscope slide supported on the slide support element an wherein the heating element can heat the reagent to a temperature of from about 90° C. to about 100° C.
2 more elements
An in situ antigen recovery and staining apparatus for treating biological samples on microscope slides, comprising:
- a slide support assembly comprising a plurality of slide support elements, each slide support element able a support a microscope slide;
- a heating element incorporated into or adjacent each slide support element for heating a microscope slide on the slide support element wherein the heating element can operate to independently heat each microscope slide disposed on the slide support assembly;
- a treatment chamber comprising a plurality of closeable reaction compartments, each reaction compartment having an openable bottom comprising a portion of the slide support element and an upper side having an upper opening;
- and
- a reagent dispenser for dispensing a reagent onto the microscope slide supported on the slide support element.
2 more elements
An in situ antigen recovery and staining apparatus for treating biological samples on microscope slides, comprising:
- a slide support assembly comprising a plurality of slide support elements, each slide support element able to support a microscope slide, wherein each slide support element is independently movable;
- a heating element incorporated into or adjacent each slide support element for heating a microscope slide on the slide support element;
- a treatment chamber comprising a plurality of closeable reaction compartments, each reaction compartment having an openable bottom comprising a portion of the slide support element and an upper side having an upper opening;
- and
- a reagent dispenser for dispensing a reagent onto the microscope slide supported on the slide support element.
2 more elements
An in situ antigen recovery and staining apparatus for treating biological samples on microscope slides, comprising:
- a slide support assembly comprising a plurality of slide support elements, each slide support element able to support a microscope slide, wherein each slide support element is independently movable;
- a heating element incorporated into or adjacent each slide support element for heating a microscope slide on the slide support element wherein the heating element can operate to independently heat each microscope slide disposed on the slide support assembly;
- a treatment chamber comprising a plurality of closeable reaction compartments, each reaction compartment having an openable bottom comprising a portion of the slide support element and an upper side having an upper opening;
- and
- a reagent dispenser for dispensing a reagent onto the microscope slide supported on the slide support element.
2 more elements
An in situ antigen recovery and staining apparatus for treating biological samples on microscope slides, comprising:
- a slide support assembly comprising a plurality of slide support elements, each slide support element able to support a microscope slide, wherein each slide support element is independently movable;
- a heating element incorporated into or adjacent each slide support element for heating a microscope slide on the slide support element wherein the heating element can operate to independently heat each microscope slide disposed on the slide support assembly;
- a treatment chamber comprising a plurality of closeable reaction compartments, each reaction compartment having an openable bottom comprising a portion of the slide support element and an upper side having an upper opening;
- and
- a reagent dispenser for dispensing reagents onto the microscope slide supported on the slide support element;
- and
- wherein the reagents can be delivered onto the microscope slides simultaneously.
4 more elements
An in situ antigen recovery and staining apparatus for treating biological samples on microscope slides, comprising:
- a slide support assembly comprising a plurality of slide support elements, each slide support element able to support a microscope slide, wherein each slide support element is independently movable;
- a heating element incorporated into or adjacent each slide support element for heating a microscope slide on the slide support element wherein the heating element can operate to independently heat each microscope slide disposed on the slide support assembly;
- a treatment chamber comprising a plurality of closeable reaction compartments, each reaction compartment having an openable bottom comprising a portion of the slide support element and an upper side having an upper opening;
- and
- a reagent dispenser for dispensing a reagent onto the microscope slide supported on the slide support element;
- and
- wherein the heating element can heat the reagent to a temperature of from about 90° C. to about 100° C.
4 more elements
An in situ antigen recovery and staining apparatus for treating biological samples on microscope slides, comprising:
- a slide support assembly comprising a plurality of slide support elements, each slide support element able to support a microscope slide, wherein each slide support element is independently movable;
- a heating element incorporated into or adjacent each slide support element for heating a microscope slide on the slide support element wherein the heating element can operate to independently heat each microscope slide disposed on the slide support assembly;
- a treatment chamber comprising a plurality of closeable reaction compartments, each reaction compartment having an openable bottom comprising a portion of the slide support element and an upper side having an upper opening;
- and
- a reagent dispenser for dispensing a reagent onto the microscope slide supported on the slide support element;
- and
- wherein the heating element can heat the reagent to a temperature of from about 90° C. to about 100° C., and
- wherein each reaction compartment can be individually vented to release steam, pressure or vapor when enclosed.
5 more elements
An in situ antigen recovery and staining apparatus for treating biological samples on microscope slides, comprising:
- a slide support assembly comprising a plurality of slide support elements, each slide support element able to support a microscope slide, wherein each slide support element is independently movable;
- a heating element incorporated into or adjacent each slide support element for heating a microscope slide on the slide support element wherein the heating element can operate to independently heat each microscope slide disposed on the slide support assembly;
- a treatment chamber comprising a plurality of closeable reaction compartments, each reaction compartment having an openable bottom comprising a portion of the slide support element and an upper side having an upper opening;
- and
- a reagent dispenser for dispensing a reagent onto the microscope slide supported on the slide support element;
- and
- wherein each microscope slide contained within the closeable reaction compartment can be heated to reach a temperature of from about 140° C. to about 160° C., and
- wherein each reaction compartment can be individually vented to release steam, pressure or vapor when enclosed.
5 more elements
Dependencies are inferred from the claim text.