Methods and interferon deficient substrates for the propagation of viruses
AssigneeMount Sinai School of Medicine of New York University
InventorsPeter Palese, Adolfo Garcia-Sastre, Robert O'Neil
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Abstract
The present invention relates, to novel methods and substrates for the propagation of viruses. The invention relates to IFN-deficient substrates and methods for propagating viruses in these unconventional substrates. In particular, the invention relates to methods of propagating viruses in immature embryonated eggs, preferably six- to nine-day-old chicken eggs. The methods of the invention are particularly attractive for growing viruses suitable for use in vaccine and pharmaceutical formulations.
Claims16
An embryonated egg six to eight days old containing a recombinantly engineered influenza virus having a mutation in the NS1 gene that diminishes or eliminates the ability of the NS1 gene product to antagonize the cellular interferon response, wherein said virus is not influenza C virus.
An embryonated egg containing in the allantoic cavity an attenuated influenza virus having a mutation in the NS1 gene that diminishes or eliminates the ability of the NS1 gene product to antagonize the cellular interferon response, wherein the embryonated egg is six to eight days old and said virus is not influenza C virus.
An embyrdnated egg six to eight days old containing delNS 1.
Dependencies are inferred from the claim text.