Production of 3-hydroxypropionic acid in recombinant organisms
AssigneeWisconsin Alumni Research Foundation
InventorsPatrick F. Suthers, Douglas C. Cameron
Life of this patent
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Abstract
The production of 3-hydroxypropionic acid (3-HP) from glycerol in a bacterial host is described. 3-HP is a useful feedstock for the production of polymeric materials. The genetic engineering of a bacterial host with two enzymes is sufficient to enable production of 3-HP. One enzyme is a glycerol dehydratase and the other is an aldehyde dehydrogenase.
Claims8
A method for producing 3-hydroxypropionic acid comprising the steps of
- providing in a fermenter a recombinant microorganism which carries a genetic construct which expresses the dhaB gene from Klebsiella pneumoniae and a gene for an aldehyde dehydrogenase, which are capable of catalyzing the production of 3-hydroxypropionic acid from glycerol;
- providing a source of glycerol or glucose for the recombinant microorganism, and
- fermenting the microorganism under conditions which result in the accumulation of 3-hydroxypropionic acid in solution in the fermenter.
A method for producing 3-hydroxypropionic acid comprising the steps of
- providing in a fermenter a recombinant microorganism which carries a genetic construct which expresses the dhaB gene from Klebsiella pneumoniae and a gene for an aldehyde dehydrogenase, which are capable of catalyzing the production of 3-hydroxypropionic acid from glycerol;
- providing a source of glycerol or glucose for the recombinant microorganism, and
- fermenting the microorganism under conditions which result in the accumulation of 3-hydroxypropionic acid wherein the gene for the aldehyde dehydrogenase is selected from the group consisting of ALDH2, ALD4, aldA and aldB.
A method for producing 3-hydroxypropionic acid comprising the steps of
- providing in a fermenter a recombinant microorganism which carries a genetic construct which expresses the dhaB gene from Klebsiella pneumoniae and a gene for an aldehyde dehydrogenase, which are capable of catalyzing the production of 3-hydroxypropionic acid from glycerol;
- providing a source of glycerol or glucose for the recombinant microorganism, and
- fermenting the microorganism under conditions which result in the accumulation of 3-hydroxypropionic acid wherein the aldehyde dehydrogenase selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6 and SEQ ID NO:8.
A recombinant E. coli host comprising in its inheritable genetic materials foreign dhaB gene from Klebsiella pneumoniae and a gene for aldehyde dehydrogenase, such that the host is capable of producing 3-hydroxypropionic acid from glycerol.
A recombinant E. coli host comprising in its inheritable genetic materials the dhaB gene from Klebsiella pneumoniae and the ald4 gene from Saccharomycetes cervisiae, such that the host is capable of producing 3-hydroxypropionic from glycerol.
A bacterial host comprising in its inheritable genetic material a genetic construction encoding for the expression of the dhaB gene from Klebsiella pneumoniae and an aldehyde dehydrogenase enzyme, such that the bacterial host is capable of converting glycerol to 3-hydroxypropionic acid.
A bacterial host comprising in its inheritable genetic material a genetic construction encoding on of a glycerol dehydratase enzyme, the amino acid sequence of which are selected from SEQ IDS NO:10, 11, 12 and 13, and an aldehyde dehydrogenase enzyme, such that the bacterial host is capable of converting glycerol to 3-hydroxypropionic acid wherein the aldehyde dehydrogenase is selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6 and SEQ ID NO:8.
A bacterial host comprising in its inheritable genetic material a genetic construction encoding for the expression of a glycerol dehydratase enzyme, the amino acid sequence of which are selected from SEQ IDS NO:10, 11, 12 and 13, and an aldehyde dehydrogenase enzyme, such that the bacterial host is capable of converting glycerol to 3-hydroxypropionic acid wherein the gene for the aldehyde dehydrogenase is selected from the group consisting of ALDH2, ALDA4, aldA and aldB.
Dependencies are inferred from the claim text.