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US 6,846,881 B2Granted
Preparation of defect-free polyacrylamide electrophoresis gels in plastic cassettes
AssigneeBio-Rad Laboratories, Inc.
InventorCory M. Panattoni
Life of this patent
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Abstract
Irregularities in the pore structures of polyacrylamide gels that are formed in cassettes against plastic walls are reduced or eliminated by the inclusion of an oxygen scavenger in the gel-forming solution. Avoidance of the irregularities results in electropherograms with fewer distortions in the solute bands.
Claims13
A method for casting a polyacrylamide gel in a plastic gel enclosure, said method comprising
- (a) forming an aqueous solution of a monomer mixture comprising acrylamide, a crosslinking agent, and an oxygen scavenger which is a member selected from the group consisting of sodium sulfite, sodium bisulfite, sodium thiosulfate, sodium lignosulfate, ammonium bisulfite, hydroquinone, diethylhydroxyethanol, diethylhydroxylamine, methylethylketoxime, ascorbic acid, erythorbic acid, and sodium erythorbate;
- and
- (b) polymerizing said monomer mixture in an uncoated plastic gel enclosure to form a polyacrylamide gel.
Dependencies are inferred from the claim text.