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US 6,846,675 B2Granted
Skin substitutes and uses thereof
AssigneeStratech Corporation
InventorsPaul Barth Conrad, Cathy Ann-Rusmussen Ivarie, Lynn Allen-Hoffmann
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Abstract
The present invention relates to in vitro cultured skin substitutes, and in particular to improved methods for organotypic culture of skin substitutes. In some embodiments, the dermal equivalent of the skin substitute is lifted to air interface of the culture prior to seeding with keratinocytes. In other embodiments, increased concentrations of collagen are used to form the dermal equivalent. In still other embodiments, optimized media are utilized to maintain the skin equivalents.
Claims14
A process for preparing a seeded dermal equivalent comprising:
- providing a dermal equivalent, keratinocytes, and culture media having an air interface;
- culturing said dermal equivalent in said culture media;
- lifting said dermal equivalent to said air interface to provide a lift dermal equivalent;
- and
- seeding said dermal equivalent with said keratinocytes to provide seeded dermal equivalent, and
- maintaining said seeded dermal equivalent at said air mt rface until said keratinocytes stratify.
3 more elements
Dependencies are inferred from the claim text.