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US 6,844,485 B2Granted
Nucleic acids encoding a phytochelatin synthase and uses thereof
AssigneeE. I. Du Pont de Nemours and Company
InventorsKarlene H. Butler, Omolayo O. Famodu, Leslie T. Harvell, Emil M. Orozco, Jr., Sonriza Rasco-Gaunt, Catherine J. Thorpe
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Abstract
The present invention relates to isolated nucleic acids encoding a polypeptide having phytochelatin synthase activity. The invention also relates to recombinant DNA constructs comprising said nucleic acids; host cells transformed with said recombinant DNA constructs, and a method for producing recombinant phytochelatin synthase in said transformed host cells.
Claims11
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a polypeptide having phytochelatin synthase activity, wherein the polypeptide has an amino acid sequence of at least 95% sequence identity, based on the ClustaIV alignment method with default pairwise alignment parameters of KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5, when compared to SEQ ID NO:18, or
- (b) a complement of the nucleotide sequence of (a), wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.
Dependencies are inferred from the claim text.