Human lineage committed cell composition with enhanced proliferative potential, biological effector function, or both; methods for obtaining same; and their uses
AssigneeAastrom Biosciences, Inc.
InventorsAlan K. Smith, Douglas M. Smith, Ramkumar K. Mandalam
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Abstract
A method for obtaining lineage committed human cells imbued with enhanced proliferative potential, biological function, or both, comprising culturing lineage committed human cells under physiologically acceptable liquid culture conditions, where the liquid culture medium is replaced at a rate and for a time sufficient to obtain the human lineage committed cells imbued with enhanced proliferative potential, biological function, or both; and isolating the cultured cells.
Claims18
A method for obtaining lineage-committed dendritic cells exhibiting enhanced biological function comprising culturing lineage committed dendritic cells ex vivo under physiologically acceptable liquid culture conditions, said conditions including replacement of the liquid culture medium at a rate of at least 25% daily replacement for more than one day and for a time sufficient to obtain lineage committed dendritic cells with enhanced biological function as compared with the biological function of the dendritic cells prior to the culturing.
A method for obtaining lineage-committed human dendritic cells exhibiting enhanced biological function comprising culturing lineage committed dendritic cells ex vivo under physiologically acceptable liquid culture conditions, said conditions including replacement of the liquid culture medium at a rate of at least 25% daily replacement for more than one day and for a time sufficient to obtain lineage committed dendritic cells with enhanced biological function as compared with the biological function of the human dendritic cells prior to the culturing.
Dependencies are inferred from the claim text.