Regulating metabolism by modifying the level of trehalose-6-phosphate
AssigneeMogen International N.V.
InventorsOscar Johannes Maria Goddijn, Jan Pen, Josephus Christianus Maria Smeekens
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Abstract
The invention involves decreasing the intracellular availability of trehalose-6-phosphate by plant cell transformation with a gene encoding trehalose-6-phosphate phosphatase from E. coli. Phenotypic effects of plant transformation with this gene include stimulation of glycolysis, cell or tissue growth, and metabolism; and inhibition of photosynthesis and bolting.
Claims20
Method for the stimulation of carbon flow in the glycolytic direction in a plant cell by decreasing the intracellular availability of trehalose-6-phosphate by transforming the plant cell with a vector that is capable of expressing the enzyme TPP of SEQ ID NO:4.
Method for the inhibition of photosynthesis in a plant cell by decreasing the intracellular availability of trehalose-6-phosphate by transforming the plant cell with a vector that is capable of expressing the enzyme TTP of SEQ ID NO:4.
Method for the stimulation of growth of a plant cell or plant tissue by decreasing the intracellular availability of trehalose-6-phosphate by transforming the plant cell or cells of the plant tissue with a vector that is capable of expressing the enzyme TPP of SEQ ID NO:4.
Method for increasing metabolism of plant cells by decreasing the intracellular availability of trehalose-6-phosphate by transforming the plant cells with a vector that is capable of expressing the enzyme TPP of SEQ ID NO:4.
Method for the stimulation of carbon flow in the glycolytic direction in a plant cell by causing expression in the plant cell of trehalose-6-phosphate phosphatase of SEQ ID NO:4.
Method for the inhibition of photosynthesis in a plant cell by causing expression in the plant cell of trehalose-6-phosphate phosphatase of SEQ ID NO:4.
Method for the stimulation of growth of a plant cell or plant tissue by causing expression in the plant cell or in cells of the plant tissue of trehalose-6-phosphate phosphatase of SEQ ID NO:4.
Method for increasing metabolism of plant cells by causing expression in the plant cells of trehalose-6-phosphate phosphatase of SEQ ID NO:4.
Method for the prevention of bolting in a plant susceptible to bolting by decreasing the intracellular availability of trehalose-6-phosphate by transforming the plant with a vector capable of expressing the enzyme TPP of SEQ ID NO:4.
Method for the prevention of bolting in a plant susceptible to bolting by causing expression in the plant of trehalose-6-phosphate phosphatase of SEQ ID NO:4.
Method for increasing the total leaf biomass of plants by transforming them with a nucleic acid sequence coding for trehalose-6-phosphate phosphatase of SEQ ID NO:4.
Method for increasing the total leaf biomass of plants by increasing the intracellular availability of trehalose-6-phosphate by transforming the plants with a vector capable of expression of the enzyme TPP of SEQ ID NO:4.
Dependencies are inferred from the claim text.