Continuous acidification demineralization process for producing osteoinductive bone; and osteoinductive bone produced thereby

A rapid demineralization process for producing osteoinductive bone, comprising subjecting bone to a continuous exchange of a predetermined volume of a demineralizing acid solution under conditions effective to produce demineralized bone wherein said volume of demineralizing acid solution is recirculated from a substantially closed container containing bone to be demineralized through an ion exchange media to remove calcium and phosphate thereby producing a regenerated acid, and said regenerated acid is returned to said substantially closed container.

A rapid demineralization process for producing osteoinductive demineralized bone, comprising placing an amount of bone to be demineralized into a substantially closed container and recirculating a predetermined volume of demineralizing acid solution from said substantially closed container through an ion exchange media to remove calcium and phosphate thereby producing a regenerated acid, and returning said regenerated acid to said substantially closed container to produce said osteoinductive demineralized bone.

A rapid demineralization process for demineralizing bone, comprising subjecting bone to a continuous exchange of a demineralized acid solution, wherein said volume of demineralizing acid solution is recirculated from a substantially closed container containing bone to be demineralized through an ion exchange media to remove calcium and phosphate thereby producing a regenerated acid, and said regenerated acid is returned to said substantially closed container, wherein said bone is demineralized until a predetermined residual calcium level in said bone is achieved.

A rapid demineralization process for producing osteoinductive bone, comprising subjecting bone to a continuous exchange of a predetermined volume of a demineralizing acid solution to produce demineralized bone, wherein said volume of demineralizing acid solution is recirculated from a substantially closed container containing bone to be demineralized through an ion exchange media to remove calcium and phosphate thereby producing a regenerated acid, and said regenerated acid is returned to said substantially closed container, wherein calcium and phosphate are continually removed from said demineralizing acid solution.

A rapid demineralization process for producing osteoinductive bone, comprising subjecting bone to a continuous exchange of a predetermined volume of a demineralizing acid solution under conditions effective to produce demineralized bone, wherein said volume of demineralizing acid solution is recirculated from a substantially closed container containing bone to be demineralized through an ion exchange media to remove calcium and phosphate thereby producing a regenerated acid, and said regenerated acid is returned to said substantially closed container, wherein calcium and phosphate are continually removed from said demineralizing acid solution.

A rapid demineralization process for producing osteoinductive bone, comprising subjecting bone to a continuous exchange of a predetermined volume of a demineralizing acid solution maintained at a constant concentration, wherein said said volume of demineralizing acid solution is recirculated from a substantially closed container containing bone to be demineralized through an ion exchange media to remove calcium and phosphate thereby producing a regenerated acid, and said regenerated acid is returned to said substantially closed container, to produce demineralized bone.