Phosphodiester α-GlcNAcase of the lysosomal targeting pathway
AssigneeGenzyme Glycobiology Research Institute, Inc.
InventorWilliam M. Canfield
Life of this patent
Estimating the term…
Abstract
The present invention provides nucleotide and amino sequences of the lysosomal targeting pathway enzyme phosphodiester α-GlcNAcase, methods of producing and methods of purifying this enzyme.
Claims24
An isolated polynucleotide, which encodes a protein comprising SEQ ID NO:6.
An isolated polynucleotide, which encodes a protein comprising amino acids 50 to 515 of SEQ iD NO:6.
An isolated polynucleotide, which comprises a nucleotide sequence that is at least 90% identical to nucleotides 151 to 1548 of SEQ ID NO:7 and encodes a protein with phosphodiester α-GlcNAcase activity.
An isolated polynucleotide, which comprises a nucleotide sequence that is at least 95% identical to nucleotides 151 to 1548 of SEQ ID NO:7 and encodes a protein with phosphodiester α-GlcNAcase activity.
A host cell comprising the isolated polynucleotide of 19.
An isolated polynucleotide, which comprises a nucleotide sequence that is at least 99% identical to nucleotides 151 to 1548 of SEQ ID NO:7 and encodes a protein with phosphodiester α-GlcNAcase activity.
A host cell comprising the isolated polynucleotide of 22.
Dependencies are inferred from the claim text.