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US 6,825,036 B2Granted
Cell usable in serum-free culture and suspension culture and process for producing virus for vaccine by using the cell
AssigneeJuridical Foundation The Chemo-Sero-Therapeutic Research Institute
InventorsKeiichi Makizumi, Kanako Masuda, Yoichiro Kino, Sachio Tokiyoshi
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Abstract
A novel MDCK-derived cell line (B-702) is provided that can be grown in the absence of fetal calf serum and in suspension culture for allowing a tank culture on the production scale and needs no carrier in suspension culture, as well as a process for producing virus for vaccine by using said cell line in a low-cost, highly safe and stable manner.
Claims3
A process for preparing an MDCK cell line that can be grown in serum-free culture and in suspension culture without a solid carrier, which comprises the steps;
- a) adapting MDCK cells to serum-free culture medium to prepare cells that can be grown in serum-free culture by a process of adjusting the medium from serum-containing medium to serum-free medium,
- b) applying the adapted MDCK cells to a suspension culture with a solid carrier to prepare cells that can be grown in suspension culture with a solid carrier;
- and
- c) applying the cells from step (b) that can be grown in suspension culture with a solid carrier to a suspension culture without a solid carrier to prepare cells that can be grown in suspension culture without need of a solid carrier.
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Dependencies are inferred from the claim text.