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US 6,825,027 B2Granted
Method of production of purified hepatitis a virus particles and vaccine preparation
AssigneeBaxter Healthcare S.A.
InventorsChrista Tauer, Heidi Meyer, Artur Mitterer, Noel Barrett
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Abstract
The present invention provides methods of purification of Hepatitis A Virus from the supernatant of an infected cell culture and production of a preparation of purified HAV antigen. The present invention is also directed to an HAV vaccine composition comprising a preparation consisting of purified mature HAV particles in an amount sufficient to induce a protective immune response in a mammal.
Claims7
A method of producing complete Hepatitis A Virus (HAV) particles comprising the steps of providing a cell culture supernatant of an HAV infected VERO cell culture;
- concentrating said concentrated cell culture supernatant;
- treating an HAV preparation from the cell culture supernatant of the HAV infected cell culture with a nucleic acid degrading agent and a protease;
- diafiltering to remove impurities to obtain a purified preparation of complete HAV particles in a single step.
Dependencies are inferred from the claim text.