Immunoassay reagent and immunoassay method
AssigneeSekisui Chemical Co., Ltd.
InventorsMasayuki Yokoi, Takayuki Akamine, Katsumi Yoshikawa
Life of this patent
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Abstract
An immunoassay reagent is obtained whereby a microscale substance such as an antigen or antibody can be assayed at a high sensitivity, and whereby a need to separate a reacted substance, e.g., an immunoreacted substance, from an unreacted substance can be eliminated or such a separation can be simplified. An immunoassay reagent, for use in the quantitative determination of a target antigen or antibody present in a sample, containing an insoluble carrier which carries an enzyme and an antibody or antigen corresponding to the antigen or antibody, an enzyme inhibitor for inhibiting the activity of the enzyme and a substrate with which the enzyme reacts.
Claims11
An immunoassay reagent for use in a quantitative determination of a target antigen or antibody present in a sample, said reagent consisting essentially of the following components:
- (a) an insoluble carrier which carries and is coupled to an enzyme and an antibody or antigen reactive with said target antigen or antibody, said insoluble carrier comprising at least one selected from the group consisting of an organic polymer powder particle, microorganism, blood cell and cell membrane fragment, said insoluble carrier being capable of aggregation;
- (b) an enzyme inhibitor for reacting with and inhibiting activity of said enzyme, said enzyme inhibitor being in a free state uncoupled to an antigen or antibody and being increasingly hindered from reacting with said enzyme when said insoluble carrier is increasingly agglutinated;
- and
- (c) a substrate for the enzyme capable of producing an optically detectable indication of reaction with the enzyme, wherein said substrate is not hindered from reacting with said enzyme when said enzyme is unreacted with said enzyme inhibitor when said insoluble carrier is increasingly aggregated, said components (a)-(c) being maintained separate and apart and mixed together only with a sample containing the target antigen or antibody.
An immunoassay reagent for use in a quantitative determination of a target antigen or antibody present in a sample, said reagent consisting essentially of the following components:
- (a) an insoluble carrier which carries and is coupled to an enzyme and an antibody or antigen reactive with said target antigen or antibody, said insoluble carrier comprising at least one selected from the group consisting of an organic polymer powder particle, microorganism, blood cell and cell membrane fragment, said insoluble carrier being capable of aggregation;
- (b) an enzyme inhibitor for reacting with and inhibiting activity of said enzyme, said enzyme inhibitor being in a free state uncoupled to an antigen or antibody and being increasingly hindered from reacting with said enzyme once said insoluble carrier is increasingly agglutinated;
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- (c) a substrate for the enzyme capable of producing an optically detectable indication of reaction with the enzyme, wherein said substrate is not hindered from reacting with said enzyme when said enzyme is unreacted with said enzyme inhibitor when said insoluble carrier is increasingly aggregated, said immunoassay reagent consisting of a first reagent and a second reagent, wherein said first reagent contains said insoluble carrier and said second reagent contains said enzyme inhibitor and said substrate.
An immunoassay reagent for use in quantitative determination of a target antigen or antibody present in a sample, said reagent consisting essentially of the following components:
- (a) an insoluble carrier which carries and is coupled to an enzyme inhibitor and an antibody or antigen reactive with said target antigen or antibody, said insoluble carrier comprising at least one selected from the group consisting of an organic polymer powder particle, microorganism, blood cell and cell membrane fragment, said insoluble carrier being capable of aggregation;
- (b) an enzyme which reacts with and whose enzymatic activity is inhibited by said enzyme inhibitor, said enzyme being in a free state uncoupled to an antigen or antibody and being increasingly sterically hindered from reacting with said enzyme inhibitor when said insoluble carrier is increasingly aggregated;
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- (c) a substrate for the enzyme capable of producing an optically detectable indication of reaction with the enzyme;
- said components (a)-(c) being maintained separate and apart and sequentially mixed together only with a sample of target antigen or antibody, the addition of the substrate facilitating reaction with the enzyme, thereby effecting an optically detectable change in absorbence.
Dependencies are inferred from the claim text.