Regulation of endogenous gene expression in cells using zinc finger proteins
AssigneeSangamo BioSciences, Inc.
InventorsGeorge Norbert Cox, III, Casey Christopher Case, Stephen P. Eisenberg, Eric Edward Jarvis, Sharon Kaye Spratt
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Abstract
The present invention provides methods for modulating expression of endogenous cellular genes using recombinant zinc finger proteins.
Claims66
A method of inhibiting expression of an endogenous cellular gene in a cell, the method comprising the step of:
- contacting a first target site in the endogenous cellular gene with a first zinc finger protein, wherein said first zinc finger protein is engineered and wherein the Kd of the zinc finger protein is less than about 25 nM;
- thereby inhibiting expression of the endogenous cellular gene.
A method of inhibiting expression of an endogenous cellular gene in a cell, the method comprising the step of:
- contacting a target site in the endogenous cellular gene with an engine fusion zinc finger protein comprising six fingers and a regulatory domain, wherein the Kd of the zinc finger protein is less than about 25 nM;
- thereby inhibiting expression of the endogenous cellular gene.
A method of activating expression of an endogenous cellular gene, the method comprising the step of:
- contacting a first target site in the endogenous cellular gene with a first zinc finger protein, wherein said first zinc finger protein is engineered and wherein the Kd of the zinc finger protein is less than about 25 nM;
- thereby activating expression of the endogenous cellular gene.
A method of activating expression of an endogenous cellular gene, the method comprising the step of:
- contacting a target site in the endogenous cellular gene with an engineered fusion zinc finger protein comprising six fingers and a regulatory domain, wherein the Kd of the zinc finger protein is less than about 25 nM;
- thereby activating expression of the endogenous cellular gene.
A method of modulating expression of an endogenous cellular gene in a cell, the method comprising the step of:
- contacting a first target site in the endogenous cellular gene with a first zinc finger protein, wherein said first zinc finger protein is engineered;
- thereby modulating expression of the endogenous cellular gene.
A method of modulating expression of an endogenous cellular gene in a cell, the method comprising the step of:
- contacting a target site in the endogenous cellular gene with an engineered fusion zinc finger protein comprising six fingers and a regulatory domain;
- thereby modulating expression of the endogenous cellular gene.
Dependencies are inferred from the claim text.