Protein L and hybrid proteins thereof
AssigneeAffitech AS
InventorsLars Björck, Ulf Sjöbring
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Abstract
The invention relates to sequences of protein L which bind to light chains of immunoglobulins. The invention also relates to hybrid proteins thereof which are able to bind to both light and heavy chains of immunoglobulin G, in particular protein LG. The invention also relates to DNA-sequences which code for the proteins, vectors which include such DNA-sequences, host cells which have been transformed with the vectors, methods for producing the proteins, reagent appliances for separation and identification of immunoglobulins, compositions and pharmaceutical compositions and pharmaceutical compositions which contain the proteins.
Claims7
An isolated protein having the ability to bind to the light chains of immunoglobulins, wherein said protein is selected from the group consisting of:
- (a) a protein consisting essentially of the amino acid sequence of SEQ ID NO:
- 1;
- (b) a protein consisting essentially of the amino acid sequence of at least one of the domains B1, B2, B3 or B4 of (a) wherein,
- (i) domain B1 is comprised of from amino acid 5 to amino acid 80 of SEQ ID NO:
- 1;
- (ii) domain B2 is comprised of from amino acid 81 to amino acid 152 of SEQ ID NO:
- 1;
- (iii) domain B3 is comprised of from amino acid 153 to amino acid 224 of SEQ ID NO:
- 1;
- (iv) domain B4 is comprised of from amino acid 225 to amino acid 296 of SEQ ID NO:
- 1;
- and
- (c) a protein consisting essentially of the sequence of multiple domains selected from one or more of the domains B1, B2, B3 and B4 of (b).
An isolated hybrid protein consisting essentially of the amino acid sequence of SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.