Thermostable creatine amidinohydrolase and process for producing the same
AssigneeKikkoman Corporation
InventorsKeisuke Furukawa, Yasuji Koyama, Masaru Suzuki
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Abstract
The present invention relates to a thermostable creatine amidinohydrolase having the following physicochemical properties: (a) hydrolyzing 1 mol of creatine to give 1 mol of sarcosine and 1 mol of urea; (b) having a substrate specificity to creatine; (c) having an optimum pH of 7.0 to 8.0; (d) having a stable pH range of 4.0 to 11.0; (e) having the optimum temperature of around 45° C.; (f) being thermostable at 53° C.; (g) having a molecular weight of 92,000 Da (as determined by gel filtration), and to a process for producing the thermostable creatine aimdinohydrolase.
Claims2
An isolated thermostable creatine amidinohydrolase that is isolated from E. coli JM109 (pUCE100B-40) (accession number FERM BP-6867), and which has the following physicochemical properties:
- (a) hydrolyzing 1 mol of creatine to give 1 mol of sarcosine and 1 mol of urea;
- (b) having a substrate specificity to creatine;
- (c) having an optimum pH of 7.0 to 8.0;
- (d) having a stable pH range of 4.0 to 11.0;
- (e) having the optimum temperature of around 45° C.;
- (f) being thermostable at 53° C.;
- (g) having a molecular weight of 92,000 Da (as determined by gel filtration).
Dependencies are inferred from the claim text.